Journal: Journal of Cellular and Molecular Medicine

Article Title: Small‐molecule peptides inhibit Z α 1 ‐antitrypsin polymerization

doi: 10.1111/j.1582-4934.2008.00608.x

Figure Lengend Snippet: MTT assay of various human cell lines treated with the Ac‐TTAI‐NH2 peptide. Five human cell lines including BEAS‐2B and NL20 normal lung epithelial cell lines, WI‐38 and IMR‐90 normal lung fibroblast cell lines, and A2058 melanoma cell line were treated for 0, 48 or 96 hrs with DMSO solvent control or 10 μM of the synthetic Ac‐TTAI‐NH2 peptide. The percentage of viable cells, relative to the solvent control‐treated cells, was measured by MTT cell proliferation assay. The results showed that no obvious cytotoxicity was detected for the Ac‐TTAI‐NH2 tetramer peptide. All experiments were performed in octuplicate in 96‐well microplates as described in the Materials and Methods section. Data are presented as mean ± S.D.

Article Snippet: Cell culture Human BEAS‐2B normal lung epithelial cells (Bioresource Collection and Research Center, Hsinchu, Taiwan) were grown in Ham’s F12 Medium (Gibco‐Invitrogen, Carlsbad, CA, USA) supplemented with 5 μg/ml insulin (Gibco‐Invitrogen), 10 μg/ml transferrin (Gibco‐Invitrogen), 0.5 μg/ml hydrocortisone (Sigma‐Aldrich, Inc., St. Louis, MO, USA), 2.5 ng/ml recombinant human epidermal growth factor (PeproTech, Rocky Hill, NJ, USA), and 12 μg/ml endothelial cell growth supplement (Millipore, Billerica, MA, USA).

Techniques: MTT Assay, MTT Cell Proliferation

Journal: Frontiers in Toxicology

Article Title: Diesel exhaust particles alter mitochondrial bioenergetics and cAMP producing capacity in human bronchial epithelial cells

doi: 10.3389/ftox.2024.1412864

Figure Lengend Snippet: Exposure of BEAS-2B cells to DEP induced oxidative stress markers and differentially affected expression of key players of the antioxidant response system.

Article Snippet: BEAS-2B cells were exposed to 100 or 300 μg/mL DEP (SRM 2975, National Institute of Standards and Technology) for 24 h. Culture medium was collected to measure IL-8 and IL-6 protein concentrations using specific enzyme-linked immunosorbent assays (ELISAs) ( ).

Techniques: Expressing, Control